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Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-QSOX1 Antibody

HPA042127
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetQSOX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-QSOX1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5768
  • Target name
    QSOX1
  • Target description
    quiescin sulfhydryl oxidase 1
  • Target synonyms
    Q6; QSCN6; quiescin Q6 sulfhydryl oxidase 1; sulfhydryl oxidase 1; testis tissue sperm-binding protein Li 62n; thiol oxidase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00391
  • Protein Name
    Sulfhydryl oxidase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Hela whole cell lysate, Rat heart tissue All lanes: QSOX1 antibody at 5.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
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Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
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Figure 1. Western blot analysis of Quiescin Q6/QSOX1 using anti-Quiescin Q6/QSOX1 antibody (A04549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Quiescin Q6/QSOX1 antigen affinity purified polyclonal antibody (Catalog # A04549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Quiescin Q6/QSOX1 at approximately 105 kDa. The expected band size for Quiescin Q6/QSOX1 is at 83 kDa.
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