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Western Blot Positive WB detected in: Hela whole cell lysate, Rat heart tissue All lanes: QSOX1 antibody at 5.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Rat heart tissue All lanes: QSOX1 antibody at 5.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Rat heart tissue All lanes: QSOX1 antibody at 5.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa

QSOX1 Antibody

CSB-PA019144LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetQSOX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    QSOX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5768
  • Target name
    QSOX1
  • Target description
    quiescin sulfhydryl oxidase 1
  • Target synonyms
    Q6; QSCN6; quiescin Q6 sulfhydryl oxidase 1; sulfhydryl oxidase 1; testis tissue sperm-binding protein Li 62n; thiol oxidase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00391
  • Protein Name
    Sulfhydryl oxidase 1
  • Scientific Description
    Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Quiescin Q6/QSOX1 using anti-Quiescin Q6/QSOX1 antibody (A04549-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Quiescin Q6/QSOX1 antigen affinity purified polyclonal antibody (Catalog # A04549-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Quiescin Q6/QSOX1 at approximately 105 kDa. The expected band size for Quiescin Q6/QSOX1 is at 83 kDa.
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