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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Anti-RACK1-25ul

HPA021676
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RACK1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB, ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63244
  • Protein Name
    Receptor of activated protein C kinase 1
  • Scientific Description
    Rabbit Polyclonal Anti-RACK1 Antibody against Human receptor for activated C kinase 1. Validated for Immunofluorescence and Western Blot
  • Reactivity
    Human, Mouse
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RACK1 using anti-RACK1 antibody (PB9243). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACK1 antigen affinity purified polyclonal antibody (Catalog # PB9243) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RACK1 at approximately 36 kDa and for RACK1 mature form at approximately 32 kDa. The expected band size for RACK1 is at 35 kDa.
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