More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Raf1 Antibody
Anti-Raf1 Antibody

Anti-Raf1 Antibody

A42606
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Raf1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.0 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to Raf1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Abbexa
C-RAF (Phospho-Tyr341) AntibodyABX012449
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RAF1 Antibody144-00223
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
  • SizePrice
Product group Antibodies
Biosynth
RAF1 antibody20R-2691
  • SizePrice
Figure 1. Western blot analysis of Raf1 using anti-Raf1 antibody (A00446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate, Lane 3: human MCF-7 whole cell lysate, Lane 4: human 22RV1 whole cell lysate, Lane 5: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raf1 antigen affinity purified polyclonal antibody (Catalog # A00446-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Raf1 at approximately 73KD. The expected band size for Raf1 is at 73KD.
Product group Antibodies
Boster Bio
Anti-Raf1 Antibody Picoband(r)A00446-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
  • SizePrice
Western Blot analysis of HeLa cells using Raf-1 Polyclonal Antibody
Product group Antibodies
Cusabio
RAF1 AntibodyCSB-PA003921
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
  • SizePrice
Product group Antibodies
Biomatik
RAF1 Polyclonal AntibodyCAC13895
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetRAF1
  • SizePrice
Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (bs-1703R) followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss
RAF1 Polyclonal AntibodyBS-1703R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetRAF1
  • SizePrice
Immunohistochemical staining of human rectum shows moderate positivity in nuclear speckles in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-RAF1 AntibodyHPA002640
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetRAF1
  • SizePrice
Raf1 antibody [N3C3] detects Raf1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. Raf1 stained by Raf1 antibody [N3C3] (GTX107763) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
Raf1 antibody [N3C3]GTX107763
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetRAF1
  • SizePrice
Product group Antibodies
MedChem Express
Raf-1 Antibody (YA107, Rabbit)HY-P80880
TargetRAF1
  • SizePrice