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Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts.

Anti-RAI14 Antibody

HPA036949
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse
TargetRAI14
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAI14
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26064
  • Target name
    RAI14
  • Target description
    retinoic acid induced 14
  • Target synonyms
    ankycorbin; ankyrin repeat and coiled-coil structure-containing protein; NORPEG; novel retinal pigment epithelial cell protein; RAI13; retinoic acid-induced protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0K7
  • Protein Name
    Ankycorbin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RAI14 using anti-RAI14 antibody (A09059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAI14 antigen affinity purified polyclonal antibody (Catalog # A09059-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAI14 at approximately 120 kDa. The expected band size for RAI14 is at 110 kDa.
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