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RAI14 Antibody (aa1-50)

LS-C762371
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetRAI14
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RAI14 Antibody (aa1-50)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:2000 - 1:10000) Immunoprecipitation Range: 2-10 µg/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26064
  • Target name
    RAI14
  • Target description
    retinoic acid induced 14
  • Target synonyms
    ankycorbin; ankyrin repeat and coiled-coil structure-containing protein; NORPEG; novel retinal pigment epithelial cell protein; RAI13; retinoic acid-induced protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAI14 using anti-RAI14 antibody (A09059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAI14 antigen affinity purified polyclonal antibody (Catalog # A09059-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAI14 at approximately 120 kDa. The expected band size for RAI14 is at 110 kDa.
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