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Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Anti-RALGAPA2 Antibody

HPA042707
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRALGAPA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RALGAPA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57186
  • Target name
    RALGAPA2
  • Target description
    Ral GTPase activating protein catalytic subunit alpha 2
  • Target synonyms
    AS250, C20orf74, bA287B20.1, dJ1049G11, dJ1049G11.4, p220, ral GTPase-activating protein subunit alpha-2, 250 kDa substrate of Akt, Ral GTPase activating protein catalytic alpha subunit 2, Ral GTPase activating protein, alpha subunit 2 (catalytic), RapGAPalpha2, akt substrate AS250, ral GTPase-activating protein alpha subunit 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ2PPJ7
  • Protein Name
    Ral GTPase-activating protein subunit alpha-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.
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