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RALGAPA2 Antibody (610-690 aa, Internal)

LS-C382149
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRALGAPA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RALGAPA2 Antibody (610-690 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000), IHC (1:100 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57186
  • Target name
    RALGAPA2
  • Target description
    Ral GTPase activating protein catalytic subunit alpha 2
  • Target synonyms
    AS250, C20orf74, bA287B20.1, dJ1049G11, dJ1049G11.4, p220, ral GTPase-activating protein subunit alpha-2, 250 kDa substrate of Akt, Ral GTPase activating protein catalytic alpha subunit 2, Ral GTPase activating protein, alpha subunit 2 (catalytic), RapGAPalpha2, akt substrate AS250, ral GTPase-activating protein alpha subunit 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.
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