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Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-RASAL1 Antibody

HPA041650
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRASAL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RASAL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8437
  • Target name
    RASAL1
  • Target description
    RAS protein activator like 1
  • Target synonyms
    RASAL, rasGAP-activating-like protein 1, GAP1 like protein, ras GTPase-activating-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95294
  • Protein Name
    RasGAP-activating-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Immunofluorescence staining of MCF-7 cells with CSB-PA019351LA01HU at 1:105, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of RASAL1 using anti-RASAL1 antibody (A06423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates Lane 2: mouse brain tissue lysates Lane 3: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL1 antigen affinity purified polyclonal antibody (Catalog # A06423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL1 at approximately 90KD. The expected band size for RASAL1 is at 90KD.
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