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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-RASAL1 Polyclonal Antibody, Unconjugated (bs-6088R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-RASAL1 Polyclonal Antibody, Unconjugated (bs-6088R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-RASAL1 Polyclonal Antibody, Unconjugated (bs-6088R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n

RASAL1 Polyclonal Antibody

BS-6088R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRASAL1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RASAL1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8437
  • Target name
    RASAL1
  • Target description
    RAS protein activator like 1
  • Target synonyms
    GAP1 like protein; ras GTPase-activating-like protein; RASAL; rasGAP-activating-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95294
  • Protein Name
    RasGAP-activating-like protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Paridis Rhizoma Sapoinins attenuates liver fibrosis in rats by regulating the expression of RASAL1/ERK1/2 signal pathway. Hong Y et al., 2016 Nov 4, J Ethnopharmacol
    Read more

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Figure 1. Western blot analysis of RASAL1 using anti-RASAL1 antibody (A06423-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates Lane 2: mouse brain tissue lysates Lane 3: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL1 antigen affinity purified polyclonal antibody (Catalog # A06423-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL1 at approximately 90KD. The expected band size for RASAL1 is at 90KD.
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