anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209)

AdipoGen Life Sciences

anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209)

10

Western Blot

Research Use Only

Polyclonal

RASGRP2

RAS guanyl releasing protein 2

calcium and DAG-regulated guanine nucleotide exchange factor I; calcium and diacylglycerol-regulated guanine nucleotide exchange factor I; CALDAG-GEFI; CDC25L; cdc25-like protein; F25B3.3 kinase-like protein; guanine exchange factor MCG7; RAS guanyl nucleotide-releasing protein 2; RAS guanyl releasing protein 2 (calcium and DAG-regulated); RAS guanyl-releasing protein 2

Rabbit

RAS guanyl-releasing protein 2

Polyclonal Antibody. Recognizes human and mouse RasGRP2 phosphorylated at pSer587 (human isoform 1), pSer586 (mouse) or pSer649 (human isoform 2). Source: Rabbit. Applications: WB. Liquid. In PBS containing 2mg/ml BSA and 0.05% sodium azide. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells. - RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.

2°C to 8°C

12352203