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Western blot: Human (5microg protein/lane) and mouse (8microg protein/lane) platelets were either not stimulated (-) or were incubated with 5microM Forskolin for 1 min (+). Western blots at the indicated dilutions were performed using anti-RasGRP2 (phosphorylated) (p
Western blot: Human (5microg protein/lane) and mouse (8microg protein/lane) platelets were either not stimulated (-) or were incubated with 5microM Forskolin for 1 min (+). Western blots at the indicated dilutions were performed using anti-RasGRP2 (phosphorylated) (p
Western blot: Human (5microg protein/lane) and mouse (8microg protein/lane) platelets were either not stimulated (-) or were incubated with 5microM Forskolin for 1 min (+). Western blots at the indicated dilutions were performed using anti-RasGRP2 (phosphorylated) (p

anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209)

Research Use Only
AG-25T-0119
AdipoGen Life Sciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetRASGRP2
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-RasGRP2 (phosphorylated) (pSer587), pAb (IG-1209)
  • Delivery Days Customer
    10
  • Antibody Specificity
    Recognizes human and mouse RasGRP2 phosphorylated at pSer587 (human isoform 1), pSer586 (mouse) or pSer649 (human isoform 2).
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Formulation
    Liquid
  • Gene ID10235
  • Target name
    RASGRP2
  • Target description
    RAS guanyl releasing protein 2
  • Target synonyms
    calcium and DAG-regulated guanine nucleotide exchange factor I; calcium and diacylglycerol-regulated guanine nucleotide exchange factor I; CALDAG-GEFI; CDC25L; cdc25-like protein; F25B3.3 kinase-like protein; guanine exchange factor MCG7; RAS guanyl nucleotide-releasing protein 2; RAS guanyl releasing protein 2 (calcium and DAG-regulated); RAS guanyl-releasing protein 2
  • Host
    Rabbit
  • Protein IDQ7LDG7
  • Protein Name
    RAS guanyl-releasing protein 2
  • Scientific Description
    Polyclonal Antibody. Recognizes human and mouse RasGRP2 phosphorylated at pSer587 (human isoform 1), pSer586 (mouse) or pSer649 (human isoform 2). Source: Rabbit. Applications: WB. Liquid. In PBS containing 2mg/ml BSA and 0.05% sodium azide. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells. - RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203