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Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.

Anti-RBFA Antibody

HPA040485
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRBFA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RBFA Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79863
  • Target name
    RBFA
  • Target description
    ribosome binding factor A
  • Target synonyms
    C18orf22, HsT169, putative ribosome-binding factor A, mitochondrial, ribosome binding factor A (putative)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N0V3
  • Protein Name
    Putative ribosome-binding factor A, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue All lanes: RBFA antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 28 kDa Observed band size: 39 kDa
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Figure 1. Western blot analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C18orf22/RBFA antigen affinity purified polyclonal antibody (Catalog # A13257-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C18orf22/RBFA at approximately 40 kDa. The expected band size for C18orf22/RBFA is at 38 kDa.
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