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RBFA Antibody (Biotin)

LS-C679292
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetRBFA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RBFA Antibody (Biotin)
  • Delivery Days Customer
    14
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID79863
  • Target name
    RBFA
  • Target description
    ribosome binding factor A
  • Target synonyms
    C18orf22, HsT169, putative ribosome-binding factor A, mitochondrial, ribosome binding factor A (putative)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (A13257-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C18orf22/RBFA antigen affinity purified polyclonal antibody (Catalog # A13257-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C18orf22/RBFA at approximately 40 kDa. The expected band size for C18orf22/RBFA is at 38 kDa.
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