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Immunohistochemical staining of human lymph node shows nuclear positivity in germinal and non germinal center.
Immunohistochemical staining of human lymph node shows nuclear positivity in germinal and non germinal center.
Immunohistochemical staining of human lymph node shows nuclear positivity in germinal and non germinal center.

Anti-RBM12 Antibody

HPA043621
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRBM12
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RBM12 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10137
  • Target name
    RBM12
  • Target description
    RNA binding motif protein 12
  • Target synonyms
    HRIHFB2091, SCZD19, SWAN, RNA-binding protein 12, SH3/WW domain anchor protein in the nucleus
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTZ6
  • Protein Name
    RNA-binding protein 12
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SWAN/RBM12 using anti-SWAN/RBM12 antibody (A13929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAN/RBM12 antigen affinity purified polyclonal antibody (Catalog # A13929) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAN/RBM12 at approximately 97 kDa. The expected band size for SWAN/RBM12 is at 97 kDa.
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