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Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human Fallopian tube shows strong nuclear positivity in glandular cells.

Anti-RBM22 Antibody

HPA001634
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRBM22
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RBM22
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55696
  • Target name
    RBM22
  • Target description
    RNA binding motif protein 22
  • Target synonyms
    Cwc2; fSAP47; functional spliceosome-associated protein 47; pre-mRNA-splicing factor RBM22; ZC3H16; zinc finger CCCH domain-containing protein 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NW64
  • Protein Name
    Pre-mRNA-splicing factor RBM22
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RBM22 using anti-RBM22 antibody (A10311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM22 antigen affinity purified polyclonal antibody (Catalog # A10311-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM22 at approximately 47 kDa. The expected band size for RBM22 is at 47 kDa.
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