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Western Blot Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate, HL60 whole cell lysate, Mouse skeletal muscle tissue All lanes: RBM22 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 42 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate, HL60 whole cell lysate, Mouse skeletal muscle tissue All lanes: RBM22 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 42 kDa Observed band size: 47 kDa
Western Blot Positive WB detected in: Raji whole cell lysate, NIH/3T3 whole cell lysate, HL60 whole cell lysate, Mouse skeletal muscle tissue All lanes: RBM22 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 42 kDa Observed band size: 47 kDa

RBM22 Antibody

CSB-PA889126LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRBM22
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Overview

  • Supplier
    Cusabio
  • Product Name
    RBM22 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55696
  • Target name
    RBM22
  • Target description
    RNA binding motif protein 22
  • Target synonyms
    Cwc2, ZC3H16, fSAP47, pre-mRNA-splicing factor RBM22, functional spliceosome-associated protein 47, zinc finger CCCH domain-containing protein 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NW64
  • Protein Name
    Pre-mRNA-splicing factor RBM22
  • Scientific Description
    Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5 splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of RBM22 using anti-RBM22 antibody (A10311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM22 antigen affinity purified polyclonal antibody (Catalog # A10311-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM22 at approximately 47 kDa. The expected band size for RBM22 is at 47 kDa.
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