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Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.

Anti-RECK Antibody

HPA071729
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRECK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RECK Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8434
  • Target name
    RECK
  • Target description
    reversion inducing cysteine rich protein with kazal motifs
  • Target synonyms
    ST15, reversion-inducing cysteine-rich protein with Kazal motifs, membrane-anchored glycoprotein (metastasis and invasion), suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs), suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs), suppressor of tumorigenicity 15 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95980
  • Protein Name
    Reversion-inducing cysteine-rich protein with Kazal motifs
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RECK using anti-RECK antibody (A06439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECK antigen affinity purified polyclonal antibody (Catalog # A06439-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RECK at approximately 110 kDa. The expected band size for RECK is at 110 kDa.
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