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RECK Antibody

CAB6718
Assay Genie
TargetRECK
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    RECK Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8434
  • Target name
    RECK
  • Target description
    reversion inducing cysteine rich protein with kazal motifs
  • Target synonyms
    ST15, reversion-inducing cysteine-rich protein with Kazal motifs, membrane-anchored glycoprotein (metastasis and invasion), suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs), suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs), suppressor of tumorigenicity 15 protein
  • Host
    Rabbit
  • Protein IDO95980
  • Protein Name
    Reversion-inducing cysteine-rich protein with Kazal motifs
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of RECK using anti-RECK antibody (A06439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECK antigen affinity purified polyclonal antibody (Catalog # A06439-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RECK at approximately 110 kDa. The expected band size for RECK is at 110 kDa.
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IHC image of CSB-PA019536LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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