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Figure 1. Western blotting validation for Anti-RECS1 TMBIM1 Antibody A10501-1 Western Blot (WB) analysis of specific cells using RECS1 polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-RECS1 TMBIM1 Antibody A10501-1 Western Blot (WB) analysis of specific cells using RECS1 polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-RECS1 TMBIM1 Antibody A10501-1 Western Blot (WB) analysis of specific cells using RECS1 polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Anti-RECS1 TMBIM1 Antibody

A10501-1
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTMBIM1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-RECS1 TMBIM1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID64114
  • Target name
    TMBIM1
  • Target description
    transmembrane BAX inhibitor motif containing 1
  • Target synonyms
    LFG3, MST100, MSTP100, PP1201, RECS1, protein lifeguard 3, transmembrane BAX inhibitor motif-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969X1
  • Protein Name
    Protein lifeguard 3
  • Scientific Description
    Boster Bio Anti-RECS1 TMBIM1 Antibody catalog # A10501-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMBIM1 using anti-TMBIM1 antibody (A10501-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMBIM1 antigen affinity purified polyclonal antibody (Catalog # A10501-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMBIM1 at approximately 35KD. The expected band size for TMBIM1 is at 35KD.
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