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anti-Resistin (human), mAb (HRES106)

AG-20B-0076
AdipoGen Life Sciences
ApplicationsELISA
Product group Antibodies
TargetRETN
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-Resistin (human), mAb (HRES106)
  • Delivery Days Customer
    10
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HRES106
  • Concentration
    1 mg/ml
  • Estimated Purity
    >95%
  • Gene ID56729
  • Target name
    RETN
  • Target description
    resistin
  • Target synonyms
    adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; cysteine-rich secreted protein A12-alpha-like 2; cysteine-rich secreted protein FIZZ3; FIZZ3; found in inflammatory zone 3; resistin; resistin delta2; RETN1; RSTN; XCP1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9HD89
  • Protein Name
    Resistin
  • Scientific Description
    Monoclonal antibody. Recognizes human resistin. Does not cross-react with mouse resistin. Applications: ELISA. Isotype: Mouse IgG2akappa. Clone: HRES106. Liquid. In PBS containing 0.02% sodium azide. The adipokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) that are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM. Resistin is secreted by obese subcutaneous adipose tissue and impaired myotube thickness and nuclear fusion by activation of the classical NF-kappaB pathway. In human, resistin levels are increased in the serum of obese individuals and resistin is used as a severity marker of asthma. - The adipokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMalpha/FIZZ 1 and RELMbeta/FIZZ 2) of FIZZ (found in inflammatory zone) that are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM. Resistin is secreted by obese subcutaneous adipose tissue and impaired myotube thickness and nuclear fusion by activation of the classical NF-kappaB pathway. In human, resistin levels are increased in the serum of obese individuals and resistin is used as a severity marker of asthma.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Resistin using anti-Resistin antibody (PB9412). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human Resistin Protein 0.5ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.
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