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Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.

Anti-RFC2 Antibody

HPA029493
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRFC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RFC2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5982
  • Target name
    RFC2
  • Target description
    replication factor C subunit 2
  • Target synonyms
    RFC40, replication factor C subunit 2, A1 40 kDa subunit, RF-C 40 kDa subunit, activator 1 subunit 2, replication factor C (activator 1) 2, 40kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35250
  • Protein Name
    Replication factor C subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RFC2 using anti-RFC2 antibody (A07724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC2 antigen affinity purified polyclonal antibody (Catalog # A07724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC2 at approximately 39 kDa. The expected band size for RFC2 is at 39 kDa.
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