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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.

Anti-RGL3 Antibody

HPA043615
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRGL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RGL3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57139
  • Target name
    RGL3
  • Target description
    ral guanine nucleotide dissociation stimulator like 3
  • Target synonyms
    ral guanine nucleotide dissociation stimulator-like 3; ralGDS-like 3; RalGEF-like protein 3, mouse homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3MIN7
  • Protein Name
    Ral guanine nucleotide dissociation stimulator-like 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RGL3 using anti-RGL3 antibody (A11774-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGL3 antigen affinity purified polyclonal antibody (Catalog # A11774-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGL3 at approximately 72 kDa. The expected band size for RGL3 is at 78 kDa.
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