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Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.

Anti-RGS18 Antibody

HPA028727
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRGS18
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RGS18
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64407
  • Target name
    RGS18
  • Target description
    regulator of G protein signaling 18
  • Target synonyms
    regulator of G-protein signaling 18; regulator of G-protein signalling 13; regulator of G-protein signalling 18; RGS13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS28
  • Protein Name
    Regulator of G-protein signaling 18
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RGS18 using anti-RGS18 antibody (A10844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS18 antigen affinity purified polyclonal antibody (Catalog # A10844-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS18 at approximately 27KD. The expected band size for RGS18 is at 27KD.
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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in megakaryocytes.
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