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Immunohistochemical staining of human bone marrow shows strong nuclear positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in megakaryocytes.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in megakaryocytes.

Anti-RGS18 Antibody

HPA045780
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRGS18
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RGS18
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64407
  • Target name
    RGS18
  • Target description
    regulator of G protein signaling 18
  • Target synonyms
    regulator of G-protein signaling 18; regulator of G-protein signalling 13; regulator of G-protein signalling 18; RGS13
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NS28
  • Protein Name
    Regulator of G-protein signaling 18
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RGS18 using anti-RGS18 antibody (A10844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS18 antigen affinity purified polyclonal antibody (Catalog # A10844-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS18 at approximately 27KD. The expected band size for RGS18 is at 27KD.
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