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Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments.
Immunohistochemical staining of human retina shows strong cytoplasmic positivity in photoreceptor segments.

Anti-RGS9 Antibody

HPA014137
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRGS9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RGS9
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8787
  • Target name
    RGS9
  • Target description
    regulator of G protein signaling 9
  • Target synonyms
    PERRS, PERRS1, RGS9L, regulator of G-protein signaling 9, regulator of G-protein signalling 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75916
  • Protein Name
    Regulator of G-protein signaling 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RGS9 using anti-RGS9 antibody (A04748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # A04748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS9 at approximately 77 kDa. The expected band size for RGS9 is at 77 kDa.
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