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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA019662ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA019662ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA019662ESR1HU at dilution of 1:100

RGS9 Antibody

CSB-PA019662ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRGS9
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Overview

  • Supplier
    Cusabio
  • Product Name
    RGS9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8787
  • Target name
    RGS9
  • Target description
    regulator of G protein signaling 9
  • Target synonyms
    PERRS, PERRS1, RGS9L, regulator of G-protein signaling 9, regulator of G-protein signalling 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75916
  • Protein Name
    Regulator of G-protein signaling 9
  • Scientific Description
    Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of RGS9 using anti-RGS9 antibody (A04748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # A04748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS9 at approximately 77 kDa. The expected band size for RGS9 is at 77 kDa.
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