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Immunohistochemical staining of human kidney shows strong membranous positivity in cells in distal tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in distal tubules.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in distal tubules.

Anti-RHBG Antibody

HPA048489
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRHBG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RHBG Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57127
  • Target name
    RHBG
  • Target description
    Rh family B glycoprotein
  • Target synonyms
    SLC42A2, ammonium transporter Rh type B, Rhesus blood group, B glycoprotein, solute carrier family 42 member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H310
  • Protein Name
    Ammonium transporter Rh type B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RHBG using anti-RHBG antibody (A05585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHBG antigen affinity purified polyclonal antibody (Catalog # A05585-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHBG at approximately 47 kDa. The expected band size for RHBG is at 49 kDa.
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