Figure 1. Western blot analysis of RHNO1 using anti-RHNO1 antibody (A13755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHNO1 antigen affinity purified polyclonal antibody (Catalog # A13755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHNO1 at approximately 40 kDa. The expected band size for RHNO1 is at 27 kDa.
Figure 1. Western blot analysis of RHNO1 using anti-RHNO1 antibody (A13755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHNO1 antigen affinity purified polyclonal antibody (Catalog # A13755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHNO1 at approximately 40 kDa. The expected band size for RHNO1 is at 27 kDa.
Anti-RHNO1 Antibody Picoband(r)
A13755
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetRHNO1
Overview
- SupplierBoster Bio
- Product NameAnti-RHNO1 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID83695
- Target nameRHNO1
- Target descriptionRAD9-HUS1-RAD1 interacting nuclear orphan 1
- Target synonymsC12orf32, HKMT1188, RHINO, RAD9, HUS1, RAD1-interacting nuclear orphan protein 1, Rad9, Rad1, Hus1 interacting nuclear orphan, rAD9, RAD1, HUS1-interacting nuclear orphan protein
- HostRabbit
- IsotypeIgG
- Scientific DescriptionBoster Bio Anti-RHNO1 Antibody Picoband® catalog # A13755. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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