Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody (M00207-1) RHOA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHOA Antibody (M00207-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Rho A + B + C Rabbit Monoclonal Antibody
M00207-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRHOA
Overview
- SupplierBoster Bio
- Product NameAnti-Rho A + B + C Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDDGF-18
- FormulationLiquid
- Gene ID387
- Target nameRHOA
- Target descriptionras homolog family member A
- Target synonymsAplysia ras-related homolog 12; ARH12; ARHA; EDFAOB; epididymis secretory sperm binding protein; oncogene RHO H12; RHO12; RHOH12; small GTP binding protein RhoA; transforming protein RhoA
- HostRabbit
- IsotypeIgG
- Protein IDP08134
- Protein NameRho-related GTP-binding protein RhoC
- Scientific DescriptionBoster Bio Anti-Rho A + B + C Rabbit Monoclonal Antibody catalog # M00207-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203