Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RhoA + RhoB + RhoC antibody [GT1228] (GTX02825) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of wide type (WT) and Rho knockout (KO) HeLa cells using GTX02825 RhoA + RhoB + RhoC antibody [GT1228]. Dilution : 1:1000 Loading : 25μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02825/CutImage_A19106_KO-WB_01_(1068999)_w_23053123_889.webp "WB analysis of wide type (WT) and Rho knockout (KO) HeLa cells using GTX02825 RhoA + RhoB + RhoC antibody [GT1228]. Dilution : 1:1000 Loading : 25μg")
![WB analysis of HUVEC whole cell lysate using GTX02825 RhoA + RhoB + RhoC antibody [GT1228]. Dilution : 1:1000 Loading : 25μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02825/CutImage_A19106_WB_01_(1069000)_w_23053123_164.webp "WB analysis of HUVEC whole cell lysate using GTX02825 RhoA + RhoB + RhoC antibody [GT1228]. Dilution : 1:1000 Loading : 25μg")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RhoA + RhoB + RhoC antibody [GT1228] (GTX02825) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02825/GTX02825_4000000372_20210122_WB_B_w_23053123_458.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RhoA + RhoB + RhoC antibody [GT1228] (GTX02825) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RhoA + RhoB + RhoC antibody [GT1228] (GTX02825) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RhoA + RhoB + RhoC antibody [GT1228]
GTX02825
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetRHOA
Overview
- SupplierGeneTex
- Product NameRhoA + RhoB + RhoC antibody [GT1228]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1228
- Concentration1.17 mg/ml
- ConjugateUnconjugated
- Gene ID387
- Target nameRHOA
- Target descriptionras homolog family member A
- Target synonymsARH12, ARHA, EDFAOB, RHO12, RHOH12, transforming protein RhoA, Aplysia ras-related homolog 12, epididymis secretory sperm binding protein, oncogene RHO H12, small GTP binding protein RhoA
- HostRabbit
- IsotypeIgG
- Protein IDP08134
- Protein NameRho-related GTP-binding protein RhoC
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![WB analysis of whole cell extracts (30 μg lysate) of Mouse Kidney (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-12 (Lane 5), Jurkat (Lane 6), and HEK-293 (Lane 7) using GTX31145 RhoA antibody [1B8-1C7]. Dilution : 1:500-1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31145/GTX31145_1853_WB_w_23060722_904.webp)
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