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Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-RICTOR Antibody

HPA037803
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRICTOR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RICTOR
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID253260
  • Target name
    RICTOR
  • Target description
    RPTOR independent companion of MTOR complex 2
  • Target synonyms
    AVO3; AVO3 homolog; hAVO3; PIA; pianissimo; rapamycin-insensitive companion of mTOR; TORC2-specific protein AVO3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6R327
  • Protein Name
    Rapamycin-insensitive companion of mTOR
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RICTOR using anti-RICTOR antibody (A03195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RICTOR antigen affinity purified polyclonal antibody (Catalog # A03195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RICTOR at approximately 230KD. The expected band size for RICTOR is at 192KD.
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