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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA108659(RICTOR Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA108659(RICTOR Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA108659(RICTOR Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)

RICTOR Antibody

CSB-PA108659
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRICTOR
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Overview

  • Supplier
    Cusabio
  • Product Name
    RICTOR Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID253260
  • Target name
    RICTOR
  • Target description
    RPTOR independent companion of MTOR complex 2
  • Target synonyms
    AVO3, PIA, hAVO3, rapamycin-insensitive companion of mTOR, AVO3 homolog, TORC2-specific protein AVO3, pianissimo
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6R327
  • Protein Name
    Rapamycin-insensitive companion of mTOR
  • Scientific Description
    Rapamycin-insensitive companion of mTOR is a protein that in humans is encoded by the RICTOR gene. RICTOR and MTOR are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth. Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RICTOR using anti-RICTOR antibody (A03195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RICTOR antigen affinity purified polyclonal antibody (Catalog # A03195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RICTOR at approximately 230KD. The expected band size for RICTOR is at 192KD.
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