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Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Anti-RILPL1 Antibody

HPA041314
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRILPL1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RILPL1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID353116
  • Target name
    RILPL1
  • Target description
    Rab interacting lysosomal protein like 1
  • Target synonyms
    GOSPEL, OPDM4, RLP1, RILP-like protein 1, GAPDH competitor of SIAH protein enhances life
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5EBL4
  • Protein Name
    RILP-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RILPL1 using anti-RILPL1 antibody (A13355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human H9C2(2-1) whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RILPL1 antigen affinity purified polyclonal antibody (Catalog # A13355-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RILPL1 at approximately 50 kDa. The expected band size for RILPL1 is at 47 kDa.
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TargetRILPL1
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TargetRILPL1
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Protein IDQ5EBL4
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