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WB analysis of rat cerebellum tissue lysate using GTX32031 RILPL1 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of rat cerebellum tissue lysate using GTX32031 RILPL1 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of rat cerebellum tissue lysate using GTX32031 RILPL1 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml

RILPL1 antibody

GTX32031
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRILPL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RILPL1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. IHC-P: 20 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID353116
  • Target name
    RILPL1
  • Target description
    Rab interacting lysosomal protein like 1
  • Target synonyms
    GOSPEL, OPDM4, RLP1, RILP-like protein 1, GAPDH competitor of SIAH protein enhances life
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5EBL4
  • Protein Name
    RILP-like protein 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RILPL1 using anti-RILPL1 antibody (A13355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human H9C2(2-1) whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RILPL1 antigen affinity purified polyclonal antibody (Catalog # A13355-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RILPL1 at approximately 50 kDa. The expected band size for RILPL1 is at 47 kDa.
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