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Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islet cells.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in islet cells.

Anti-RIN1 Antibody

HPA035491
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRIN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RIN1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9610
  • Target name
    RIN1
  • Target description
    Ras and Rab interactor 1
  • Target synonyms
    ras and Rab interactor 1, ras inhibitor 1, ras inhibitor JC99, ras inhibitor RIN1, ras interaction/interference protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13671
  • Protein Name
    Ras and Rab interactor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Immunofluorescence staining of Hela cells with CSB-PA019726LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of RIN1 using anti-RIN1 antibody (A04953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIN1 antigen affinity purified polyclonal antibody (Catalog # A04953-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIN1 at approximately 90 kDa. The expected band size for RIN1 is at 84 kDa.
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