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Immunohistochemical staining of human Endometrium shows strong nuclear positivity in glandular cells and cells in endometrial stroma.
Immunohistochemical staining of human Endometrium shows strong nuclear positivity in glandular cells and cells in endometrial stroma.
Immunohistochemical staining of human Endometrium shows strong nuclear positivity in glandular cells and cells in endometrial stroma.

Anti-RING1 Antibody

HPA008701
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRING1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RING1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6015
  • Target name
    RING1
  • Target description
    ring finger protein 1
  • Target synonyms
    E3 ubiquitin-protein ligase RING1; polycomb complex protein RING1; really interesting new gene 1 protein; RING1A; RING-type E3 ubiquitin transferase RING1; RNF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06587
  • Protein Name
    E3 ubiquitin-protein ligase RING1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RING1 using anti-RING1 antibody (A01824-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RING1 antigen affinity purified polyclonal antibody (Catalog # A01824-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RING1 at approximately 50 kDa. The expected band size for RING1 is at 43 kDa.
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