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Anti-RNASE3 Antibody

144-63307
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetRNASE3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-RNASE3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID6037
  • Target name
    RNASE3
  • Target description
    ribonuclease A family member 3
  • Target synonyms
    cytotoxic ribonuclease; ECP; eosinophil cationic protein; RAF1; ribonuclease 3; ribonuclease, RNase A family, 3; RNase 3; RNS3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12724
  • Protein Name
    Eosinophil cationic protein
  • Scientific Description
    RNASE3 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribonuclease 3 antigen affinity purified polyclonal antibody (Catalog # A03115-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribonuclease 3 at approximately 18KD. The expected band size for Ribonuclease 3 is at 18KD.
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