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Western Blot analysis of 293 cells using ECP Polyclonal Antibody
Western Blot analysis of 293 cells using ECP Polyclonal Antibody
Western Blot analysis of 293 cells using ECP Polyclonal Antibody

RNASE3 Antibody

CSB-PA002246
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRNASE3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNASE3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6037
  • Target name
    RNASE3
  • Target description
    ribonuclease A family member 3
  • Target synonyms
    ECP, RAF1, RNS3, eosinophil cationic protein, RNase 3, cytotoxic ribonuclease, ribonuclease 3, ribonuclease, RNase A family, 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12724
  • Protein Name
    Eosinophil cationic protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ribonuclease 3 using anti-Ribonuclease 3 antibody (A03115-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribonuclease 3 antigen affinity purified polyclonal antibody (Catalog # A03115-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribonuclease 3 at approximately 18KD. The expected band size for Ribonuclease 3 is at 18KD.
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