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Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.

Anti-RNF138 Antibody

HPA041101
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRNF138
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF138
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51444
  • Target name
    RNF138
  • Target description
    ring finger protein 138
  • Target synonyms
    E3 ubiquitin-protein ligase RNF138; hNARF; HSD-4; NARF; nemo-like kinase associated ring finger protein; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; ring finger protein 138, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF138; STRIN; testicular tissue protein Li 165
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WVD3
  • Protein Name
    E3 ubiquitin-protein ligase RNF138
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSD-4/RNF138 using anti-HSD-4/RNF138 antibody (A09061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD-4/RNF138 antigen affinity purified polyclonal antibody (Catalog # A09061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD-4/RNF138 at approximately 28 kDa. The expected band size for HSD-4/RNF138 is at 28 kDa.
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Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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