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WB analysis of mouse multiple (A) whole cell lysates using GTX56117 RNF138 antibody.
WB analysis of mouse multiple (A) whole cell lysates using GTX56117 RNF138 antibody.
WB analysis of mouse multiple (A) whole cell lysates using GTX56117 RNF138 antibody.

RNF138 antibody

GTX56117
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRNF138
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Overview

  • Supplier
    GeneTex
  • Product Name
    RNF138 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51444
  • Target name
    RNF138
  • Target description
    ring finger protein 138
  • Target synonyms
    E3 ubiquitin-protein ligase RNF138; hNARF; HSD-4; NARF; nemo-like kinase associated ring finger protein; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; ring finger protein 138, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF138; STRIN; testicular tissue protein Li 165
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WVD3
  • Protein Name
    E3 ubiquitin-protein ligase RNF138
  • Scientific Description
    The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSD-4/RNF138 using anti-HSD-4/RNF138 antibody (A09061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD-4/RNF138 antigen affinity purified polyclonal antibody (Catalog # A09061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD-4/RNF138 at approximately 28 kDa. The expected band size for HSD-4/RNF138 is at 28 kDa.
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