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Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows nuclear positivity in urothelial cells.

Anti-RNF2 Antibody

HPA026803
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6045
  • Target name
    RNF2
  • Target description
    ring finger protein 2
  • Target synonyms
    BAP-1, BAP1, DING, HIPI3, LUSYAM, RING1B, RING2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, RING finger protein 1B, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, huntingtin-interacting protein 2-interacting protein 3, protein DinG
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99496
  • Protein Name
    E3 ubiquitin-protein ligase RING2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RING2/RING1B/RNF2 antigen affinity purified polyclonal antibody (Catalog # A01209-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RING2/RING1B/RNF2 at approximately 38 kDa. The expected band size for RING2/RING1B/RNF2 is at 38 kDa.
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