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ProductsBack/RNF2 / RING2 / RING1B Antibody

RNF2 / RING2 / RING1B Antibody

LS-C334144
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetRNF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RNF2 / RING2 / RING1B Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Applications Supplier
    ChIP-Seq (1:20 - 1:100), IF (1:50 - 1:200), IHC (1:50 - 1:200), IP (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.69 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6045
  • Target name
    RNF2
  • Target description
    ring finger protein 2
  • Target synonyms
    BAP1; BAP-1; DING; E3 ubiquitin-protein ligase RING2; HIP2-interacting protein 3; HIPI3; huntingtin-interacting protein 2-interacting protein 3; LUSYAM; protein DinG; RING finger protein 1B; RING finger protein BAP-1; RING1B; RING2; RING-type E3 ubiquitin transferase RING2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RING2/RING1B/RNF2 using anti-RING2/RING1B/RNF2 antibody (A01209-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RING2/RING1B/RNF2 antigen affinity purified polyclonal antibody (Catalog # A01209-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RING2/RING1B/RNF2 at approximately 38 kDa. The expected band size for RING2/RING1B/RNF2 is at 38 kDa.
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