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Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.

Anti-RNF212 Antibody

HPA057527
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRNF212
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RNF212
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID285498
  • Target name
    RNF212
  • Target description
    ring finger protein 212
  • Target synonyms
    probable E3 SUMO-protein ligase RNF212; probable E3 SUMO-protein transferase RNF212; ZHP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ495C1
  • Protein Name
    Probable E3 SUMO-protein ligase RNF212
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF212 using anti-RNF212 antibody (A11605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF212 antigen affinity purified polyclonal antibody (Catalog # A11605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF212 at approximately 22 kDa. The expected band size for RNF212 is at 33 kDa.
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