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Western blot All lanes: RNF212 antibody at 14ug/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 15, 22, 32, 27, 31 kDa Observed band size: 34 kDa
Western blot All lanes: RNF212 antibody at 14ug/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 15, 22, 32, 27, 31 kDa Observed band size: 34 kDa
Western blot All lanes: RNF212 antibody at 14ug/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 15, 22, 32, 27, 31 kDa Observed band size: 34 kDa

RNF212 Antibody

CSB-PA019872ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRNF212
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNF212 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID285498
  • Target name
    RNF212
  • Target description
    ring finger protein 212
  • Target synonyms
    probable E3 SUMO-protein ligase RNF212; probable E3 SUMO-protein transferase RNF212; ZHP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ495C1
  • Protein Name
    Probable E3 SUMO-protein ligase RNF212
  • Scientific Description
    SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF212 using anti-RNF212 antibody (A11605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF212 antigen affinity purified polyclonal antibody (Catalog # A11605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF212 at approximately 22 kDa. The expected band size for RNF212 is at 33 kDa.
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