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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Anti-ROCK1 Antibody

HPA045639
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetROCK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ROCK1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6093
  • Target name
    ROCK1
  • Target description
    Rho associated coiled-coil containing protein kinase 1
  • Target synonyms
    p160 ROCK-1; P160ROCK; renal carcinoma antigen NY-REN-35; rho-associated protein kinase 1; ROCK-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13464
  • Protein Name
    Rho-associated protein kinase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (Catalog # A00722-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 160 kDa. The expected band size for ROCK1 is at 158 kDa.
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