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Western Blot analysis of COS7 cells using Rock-1 Polyclonal Antibody
Western Blot analysis of COS7 cells using Rock-1 Polyclonal Antibody
Western Blot analysis of COS7 cells using Rock-1 Polyclonal Antibody

ROCK1 Antibody

CSB-PA004017
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetROCK1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ROCK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6093
  • Target name
    ROCK1
  • Target description
    Rho associated coiled-coil containing protein kinase 1
  • Target synonyms
    p160 ROCK-1; P160ROCK; renal carcinoma antigen NY-REN-35; rho-associated protein kinase 1; ROCK-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13464
  • Protein Name
    Rho-associated protein kinase 1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (Catalog # A00722-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 160 kDa. The expected band size for ROCK1 is at 158 kDa.
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