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Immunohistochemical staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human colorectal cancer shows strong cytoplasmic positivity in tumor cells.

Anti-ROCK2 Antibody

HPA007459
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetROCK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ROCK2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9475
  • Target name
    ROCK2
  • Target description
    Rho associated coiled-coil containing protein kinase 2
  • Target synonyms
    ROCK-II, rho-associated protein kinase 2, p164 ROCK-2, rho-associated, coiled-coil-containing protein kinase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75116
  • Protein Name
    Rho-associated protein kinase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (A01023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7 : rat brain tissue lysates, Lane 8 : rat PC-12 whole cell lysates, Lane 9 : mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # A01023-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161KD. The expected band size for ROCK2 is at 161KD.
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