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Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.
Immunofluorescent staining of human cell line HeLa shows localization to nuclear bodies.

Anti-RORC Antibody

HPA065620
Atlas Antibodies
ApplicationsImmunoCytoChemistry, Chip-exoSeq
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRORC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RORC
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, Chip-exoSeq
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6097
  • Target name
    RORC
  • Target description
    RAR related orphan receptor C
  • Target synonyms
    IMD42; NR1F3; nuclear receptor ROR-gamma; nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F member 3; RAR-related orphan nuclear receptor variant 2; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; RORG; RZRG; RZR-GAMMA; TOR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51449
  • Protein Name
    Nuclear receptor ROR-gamma
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROR gamma/RORC using anti-ROR gamma/RORC antibody (A00444-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR gamma/RORC antigen affinity purified polyclonal antibody (Catalog # A00444-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR gamma/RORC at approximately 58 kDa. The expected band size for ROR gamma/RORC is at 58 kDa.
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