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ProductsBack/Goat anti-RORC (aa200-212)

Goat anti-RORC (aa200-212)

EB10749
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetRORC
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RORC (aa200-212) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6097
  • Target name
    RORC
  • Target description
    RAR related orphan receptor C
  • Target synonyms
    IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, nuclear receptor ROR-gamma, nuclear receptor RZR-gamma, nuclear receptor subfamily 1 group F member 3, retinoic acid-binding receptor gamma, retinoid-related orphan receptor gamma
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005051.2; NP_001001523.1. GeneIDs all Nonhuman: 19885 (mouse);. Purification: Antigen affinity purified. Names and symbols: RORC; RAR-related orphan receptor C; MGC129539; NR1F3; RORG; RZR-GAMMA; RZRG; TOR; RAR-related orphan receptor C, isoform a; RAR-related orphan receptor gamma; nuclear receptor ROR-gamma; nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F me
  • Reactivity Supplier
    Human, Mouse, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROR gamma/RORC using anti-ROR gamma/RORC antibody (A00444-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR gamma/RORC antigen affinity purified polyclonal antibody (Catalog # A00444-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR gamma/RORC at approximately 58 kDa. The expected band size for ROR gamma/RORC is at 58 kDa.
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