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Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.

Anti-RPGRIP1L Antibody

HPA039405
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRPGRIP1L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RPGRIP1L Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23322
  • Target name
    RPGRIP1L
  • Target description
    RPGRIP1 like
  • Target synonyms
    COACH3, CORS3, FTM, JBTS7, MKS5, NPHP8, PPP1R134, protein fantom, RPGR-interacting protein 1-like protein, fantom homolog, nephrocystin-8, protein phosphatase 1, regulatory subunit 134
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68CZ1
  • Protein Name
    Protein fantom
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPGRIP1L using anti-RPGRIP1L antibody (A04178-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPGRIP1L antigen affinity purified polyclonal antibody (Catalog # A04178-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPGRIP1L at approximately 151 kDa. The expected band size for RPGRIP1L is at 151 kDa.
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